Complex-formation between reduced xanthine oxidase and purine substrates demonstrated by electron paramagnetic resonance
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Complex-formation between reduced xanthine oxidase and purine substrates demonstrated by electron paramagnetic resonance.
The origin of the Rapid molybdenum electron-paramagnetic-resonance signals, which are obtained on reducing xanthine oxidase with purine or with xanthine, and whose parameters were measured by Bray & Vänngård (1969), was studied. It is concluded that these signals represent complexes of reduced enzyme with substrate molecules. Xanthine forms one complex at high concentrations and a different one...
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I M. D. KAMEN, R. G. BARTSCH, T. HORIO AND H. DE KLERK, in S. P. COLO\VICK AND N. O. tXAPLAN, Methods in Enzymology, Vol. VI, Academic Press, New York, I963, p. 391. 2 T. HORIO AND M. D. KAMEN, Biochim. Biophys. Acta, 48 (I96I) 266. 3 G. COHEN-BAZIRE, ~V. R. SISTROM AND R. Y. STANIER, J . Cellular Physiol., 49 (1957) 25. 4 R. G. BARTSCH, in H. (;EST AND A. SAN PIETRO, Bacterial Photosynthesis, ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1969
ISSN: 0306-3283
DOI: 10.1042/bj1140735